Metabolic Flux Analysis

Pathway analysis using metabolic flux provides detailed mechanistic information, which is enabled by the combination of stable isotopes and sensitive detection using advanced analytical methods. Flux analysis using stable isotopes such as 2H. 13C and 15N provides a unique opportunity for establishing metabolic routes unambiguously and determining the rates at which metabolites are produced or consumed. Due to the detailed information provided, metabolite flux analysis is increasingly of interest in metabolic studies. Cells or animal models can be supplied with 2H, 13C or 15N isotope-labeled substrates and the formed labeled metabolites are analyzed to derive information on pathways and fluxes. Both NMR and MS methods are used in the flux analysis.

Our main center at SLU provides metabolic flux analysis services using NMR and MS methods employing global or targeted methods.For additional information about costs, please check assays & fees.

GC-MS flux analysis
Isotope tracing and metabolic flux analysis workflow

Isotope chemical derivatization tracer analysis of primary metabolism including TCA cycle, glycolysis, amino acids, fatty acids, etc.

References

      • Du, J.; Rountree, A.; Cleghorn, W. M.; Contreras, L.; Lindsay, K. J.; Sadilek, M.; Gu, H.; Djukovic, D.; Raftery, D.; Satrústegui, J.; et al. Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina. J. Biol. Chem. 2016, 291 (9), 4698–4710. https://doi.org/10.1074/jbc.M115.698985.
NMR flux analysis
labelled
Quantitative label incorporation and NMR metabolic flux analysis

Isotope tracer analysis of primary metabolism without the need of chemical derivatization.

References

      • Kalyuzhnaya; M. G.; Yang; S.; Rozova; O. N.; Smalley; N. E.; Clubb; J.; Lamb; A.; Gowda; G. A.; Raftery; D.; Fu; Y.; Bringel; F.; Vuilleumier; S.; Beck; D. A.; Trotsenko; Y. A.; Khmelenina; V. N.; Lidstrom; M. E. Highly efficient methane biocatalysis revealed in a methanotrophic bacterium. Nat. Commun. 2013, 4, 2785. https://doi.org/10.1038/ncomms3785.