Assays & Fees

NW-MRC Assays and Fees (as of 11/1/2019)

Assay NameAssay DescriptionPlatformMetabolitesUWFH*Non-UWInternationalCommercial Comments
Targeted 300
Targeted Assay of 300+ Aqueous Metabolites
Shimadzu LC/Sciex Triple Quad 6500+ MS
~300 metabolites in ~60 pathways$105$113$147$169$225Aqueous metabolites
LipidyzerTargeted Lipidomics of 1000+ LipidsAB Sciex QTRAP 5500 with SelexION~800 lipids from 13 different lipid classes
$114$122$158$182$243Absolute Quantitation
Global LC-MS

Aqueous Global Profiling
Agilent 6520 Q-TOF LC/MS~1000 MS features, ~400 metabolite IDs
$110$118$153$176$235Aqueous metabolites
Lipid Global Profiling
Agilent 6520 Q-TOF LC/MS~1000 MS features, ~400 lipids
$110$118$153$176$235Lipids
GC-MS

GC-MS Profiling
Agilent 5975C GC/MSD>600 MS features, ~100-200 metabolite IDs
$78$84$109$125$167MSTFA or MTBSTFA derivatization
GC-MS Isotopic Analysis
Agilent 5975C GC/MSD
TCA, Glycolysis, Amino Acids, Fatty Acids, etc.
$78$84$109$125$167MTBSTFA derivatization
Targeted PathwaysCarnitine Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~40 carnitines
$71$76$98$113$151Acyl Carnitines C2-C18
Bile Acid Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~30 bile acids
$71$76$98$113$151Cholic, glycocholic, taurocholic acids, etc.
Cardiolipin Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~20 CPs
$71$76$98$113$151CP(18:2-18:2)-(18:2-18:2), CP(18:3-18:2)-(18:2-18:2), etc.
Tryptophan Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~20 tryptophan metabolites
$71$76$98$113$151Serotonin, trp, kynurenine, kynurenic acid, etc.
Protein Hydrolysis Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S microAmino Acids
$71$76$98$113$151HCl hydrolysis
Few MetabolitesTargeted analysis of a few metabolites
AB Sciex QTRAP 5500 or Waters TQS-Micro
1 to ~ 5 metabolites
$52$55$72$83$110Metabolites by request and assumes sufficient samples to analyze
Global NMR
NMR Data Collection and Analysis
Bruker 800 MHz
~50 metabolites
$104$111$144$166$221Profiling and targeted
Assay Development
New Assay Method Development
Any platform in NW-MRC
Customized metabolite assay analysis development
$125$134$174$200$267Added to the sample analysis fee, including unknown identification
Data AnalysisMultivariate Statistical and Pathway Analysis
Matlab, R, PLS-Toolbox, etc.
$91$97$126$146$194PCA, PLS-DA, SVM, etc.
Sample Prep

Total Protein Content
BCA Assay or ELISA Kits
$14$14$19$22$29
Sample Preparation
$14$14$19$22$29For any type of biofluid analysis
Tissue PrepTissue Preparation
$26$28$36$42$55For any type of tissue analysis

Notes: Prices shown for all non-UW sites include 6.85% overhead. Prices for samples from Fred Hutch are the same as the UW except for 6.85% overhead.