Assays & Fees

NW-MRC Assays and Fees (as of 11/1/2019)

Assay NameAssay DescriptionPlatformMetabolitesUWFH*Non-UWInternationalCommercial Comments
Targeted 300
Targeted Assay of 300+ Aqueous Metabolites
Shimadzu LC/Sciex Triple Quad 6500+ MS
~300 metabolites in ~60 pathways$83$88$115$132$176Aqueous metabolites
LipidyzerTargeted Lipidomics of 1000+ LipidsAB Sciex QTRAP 5500 with SelexION~800 lipids from 13 different lipid classes
$92$98$127$147$196Absolute Quantitation
Global LC-MS

Aqueous Global Profiling
Agilent 6520 Q-TOF LC/MS~1000 MS features, ~400 metabolite IDs
$102$109$141$163$217Aqueous metabolites
Lipid Global Profiling
Agilent 6520 Q-TOF LC/MS~1000 MS features, ~400 lipids
$102$109$141$163$217Lipids
GC-MS

GC-MS Profiling
Agilent 5975C GC/MSD>600 MS features, ~100-200 metabolite IDs
$58$62$81$94$125MSTFA or MTBSTFA derivatization
GC-MS Isotopic Analysis
Agilent 5975C GC/MSD
TCA, Glycolysis, Amino Acids, Fatty Acids, etc.
$58$62$81$94$125MTBSTFA derivatization
Targeted PathwaysCarnitine Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~40 carnitines
$41$44$57$65$87Acyl Carnitines C2-C18
Bile Acid Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~30 bile acids
$41$44$57$65$87Cholic, glycocholic, taurocholic acids, etc.
Cardiolipin Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~20 CPs
$41$59
$89
$103
$137
CP(18:2-18:2)-(18:2-18:2), CP(18:3-18:2)-(18:2-18:2), etc.
Tryptophan Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S micro~20 tryptophan metabolites
$41$59
$89
$103
$137
Serotonin, trp, kynurenine, kynurenic acid, etc.
Protein Hydrolysis Analysis
Waters Acquity UPLC I-Class/Xevo TQ-S microAmino Acids
$41$59
$89
$103
$137
HCl hydrolysis
Few MetabolitesTargeted analysis of a few metabolites
AB Sciex QTRAP 5500 or Waters TQS-Micro
1 to ~ 5 metabolites
$31$33$43$49$66Metabolites by request and assumes sufficient samples to analyze
Global NMR
NMR Data Collection and Analysis
Bruker 800 MHz
~50 metabolites
$90$96$125$144$192Profiling and targeted
Assay Development
New Assay Method Development
Any platform in NW-MRC
Customized metabolite assay analysis development
$100$107$139$161$214Added to the sample analysis fee, including unknown identification
Data AnalysisMultivariate Statistical and Pathway Analysis
Matlab, R, PLS-Toolbox, etc.
$71$76$99$114$152PCA, PLS-DA, SVM, etc.
Sample Prep

Total Protein Content
BCA Assay or ELISA Kits
$14$15$20$23$30
Sample Preparation
$14$15$20$23$30For any type of biofluid analysis
Tissue PrepTissue Preparation
$20$22$28$32$43For any type of tissue analysis

Notes: Prices shown for all non-UW sites include 6.85% overhead. Prices for samples from Fred Hutch are the same as the UW except for 6.85% overhead.