UW-SLU Mass Spectrometry Resources
Sciex Triple Quad 6500+ / Shimadzu Nexera 20XR HPLC
This LC-MS system is installed for high throughput metabolic analysis, with two LC columns that alternate between separation and reconditioning. The Sciex Triple Quad 6500+ mass spectrometer is equipped with ESI Turbo Ionspray and APCI sources. This system is ideal for metabolite quantitation. The system can detect over 300 metabolites.
AB Sciex QTRAP 5500 MS / Shimadzu LC-30 HPLC
This instrument includes a Sciex Lipidzyer platform that uses SelexION differential mobility spectrometry (DMS) and is capable of measuring the absolute concentrations of more than 1100 lipids from 13 different lipid classes. It is also used to run a new oxylipin assay.
Waters Xevo TQ-S Micro MS
This instrument is equipped with an Aquity UPLC system and HILIC or reverse phase chromatography columns for either aqueous or lipid metabolomics analysis. The instrument is used for a variety of metabolite assays such as a recently developed 55 bile acid assay, as well as the development of globally optimized targeted mass spectrometry (GOT-MS), an approach we have introduced that maximizes the metabolite coverage using a targeted LC-MS/MS system.
Agilent 6520 Q-TOF / Agilent 1200 LC
The Agilent 6520 Q-TOF is used for global metabolic profiling of aqueous metabolites and lipids (lipidomics) and provides high sensitivity, high resolution, and high mass accuracy. The Q-TOF MS is set up with both ESI and APCI ion interfaces. It also can perform MS/MS experiments using a quadrupole, a hexapole collision cell, and a time-of-flight analyzer to produce high-resolution spectra.
Agilent 7890A / 5875C GC-MS
This system is used to analyze volatile and semi-volatile metabolites, and those after derivatization. The Agilent 7890A GC has enhanced separation capabilities, improved productivity, and real-time self-monitoring instrument intelligence. EI spectra are quite reproducible, and thus the GC-MS system can easily identify metabolites by comparing the spectra of samples against those from the commercial NIST library. The Agilent 5875C is a quadruple MS detector and it has excellent performance for quantitative purposes.