Aqueous Metabolite Analysis

Targeted Assay of 300+ Aqueous Metabolites
Relative quantitation of 300+ aqueous metabolites in both positive and negative ionization modes from 42 KEGG–defined metabolic pathways, along with ~30 stable-isotope labeled internal standards for concentration determinations. The list of metabolic pathways and the number of metabolites (in parentheses) accessed via this method:

  • 2-oxocarboxylic acid metabolism (22)
  • Alanine, aspartate and glutamate metabolism (16)
  • Amino Acid Biosynthesis (56)
  • Arginine Biosynthesis (11)
  • Arginine and proline metabolism (22)
  • Beta-alanine metabolism (11)
  • Biotin metabolism (2)
  • Butanoate metabolism (13)
  • Citrate cycle (TCA cycle) (13)
  • Cysteine and methionine metabolism (23)
  • Fatty acid metabolism (9)
  • Glutathione metabolism (14)
  • Glycine, serine and threonine metabolism (23)
  • Glycolysis / Gluconeogenesis (15)
  • Glyoxalate and Dicarboxylate metabolism (18)
  • Histidine metabolism (12)
  • Ketone bodies synthesis and degradation (4)
  • Lipids pathway (15 Pathways) (25)
  • Lysine biosynthesis (6)
  • Lysine degradation (13)
  • Microbial metabolism (73)
  • Nicotinate and nicotinamide metabolism (15)
  • Nitrogen metabolism (10)
  • Nucleotide metabolism (48)
  • Oxidative phosphorylation (7)
  • Pentose and glucoronate interconverstions (10)
  • Pentose phosphate pathway (13)
  • Phenylalanine metabolism (22)
  • Phenylalanine, tyrosine and tryptophan biosynthesis (12)
  • Phosphonate and phosphinate metabolism (4)
  • Propanoate metabolism (12)
  • Purine metabolism (29)
  • Pyrimidine metabolism (32)
  • Pyruvate metabolism (10)
  • Sulfur Metabolism (7)
  • Taurine and hypotaurine metabolism (6)
  • Tryptophan metabolism (14)
  • Tyrosine metabolism (23)
  • Urea Cycle (7)
  • Valine, leucine and isoleucine biosynthesis (12)
  • Valine, leucine and isoleucine degradation (10)
  • Vitamins (13)

References

    • Navarro, S.L.; Tarkhan, A.; Shojaie, A.; Randolph, T.W.; Gu, H.; Djukovic, D.; Osterbauer, K.J.; Hullar, M.A.; Kratz, M.; Neuhouser, M.L.; Lampe, P.D.; Raftery, D.; Lampe, J.W. Plasma metabolomics profiles suggest beneficial effects of a low-glycemic load dietary pattern on inflammation and energy metabolism. Am J Clin Nutr. 2019, 110 (4), 984-992. https://doi.org/10.1093/ajcn/nqz169.
    • Skill, N. J.; Elliott, C.M.; Ceballos, B.; Saxena, R.; Pepin, R.; Bettcher, L.; Ellensberg, M.; Raftery, D.; Maluccio, M. A.;  Ekser, B.; Mangus, R. S.; Kubal, C. A. Metabolomic Characterization of Human Model of Liver Rejection Identifies Aberrancies Linked to Cyclooxygenase (COX) and Nitric Oxide Synthase (NOS), Ann Transplant. 2019, 24, 341-349. https://doi.org/10.12659/AOT.913800.
    • Zhu, J.; Djukovic, D.; Deng, L.; Gu, H.; Himmati, F.; Chiorean, E. G.; Raftery, D. Colorectal cancer detection using targeted serum metabolic profiling. J Proteome Res. 2014, 13 (9), 4120-4130. https://doi.org/10.1021/pr500494u.

Quantitative NMR Analysis
Absolute quantitation of 60+ metabolites in bio-fluids, cells and tissue extracts. The metabolites quantitated represent various classes including amino acids, organic acids, carbohydrates, and heterocyclic compounds. Following is the list of metabolites targeted in the analysis.

  • 1-Methylhistidine
  • Choline
  • Leucine
  • 1,2-Propanediol
  • Citric acid
  • Lysine
  • Creatine
  • Mannose
  • 2-Hydroxybutyric acid
  • Creatinine
  • Methanol
  • 2-Hydroxyisovaleric acid
  • Dimethylamine
  • Methionine
  • 2-Aminobutyric acid
  • Dimethylglycine
  • Myoinositol
  • 2-Propanol
  • Ethanol
  • Ornithine
  • 2-Oxoisocaproic acid
  • Formic acid
  • Phenylalanine
  • Fumaric acid
  • Proline
  • 2-Oxoisovaleric acid
  • Glucose
  • Pyroglutamic acid
  • 3-Hydroxybutyric acid
  • Sarcosine
  • 3-Hydroxyisovaleric acid
  • Glutamic acid
  • Serine
  • 3-Methylhistidine
  • Glutamine
  • Succinic acid
  • 3-Methyl-2-oxovaleric acid
  • Glycerol
  • Sucrose
  • Acetic acid
  • Glycine
  • Threonine
  • Acetone
  • Hippuric acid
  • Tryptophan
  • Acetylcarnitine
  • Histidine
  • N-Acetylglycine
  • Hypoxanthine
  • Tyrosine
  • Alanine
  • Isobutyric acid
  • Urea
  • Arginine
  • Isoleucine
  • Uridine
  • Asparagine
  • Valine
  • Aspartic acid
  • Isovaleric acid
  • Xanthine
  • Benzoic acid
  • Lactic acid
  • Betaine
  • Carnitine

References

    • Nagana Gowda, G. A.; Gowda, Y. N.; Raftery, D. Expanding the Limits of Human Blood Metabolite Quantitation Using NMR Spectroscopy. Anal. Chem. 2015, 87 (1), 706–715. https://doi.org/10.1021/ac503651e.
    • Nagana Gowda, G. A.; Raftery, D. Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy. Anal. Chem. 2014, 86 (11), 5433-5440. https://doi.org/10.1021/ac5005103.